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custom sgrna and shrna libraries  (Cellecta Inc)

 
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    Cellecta Inc custom sgrna and shrna libraries
    ( A ) Scramble, MTOR and PIK3C3 sgRNAs were introduced into H4 Cas9 GFP-SQSTM1 cells by lentiviral infection and GFP fluorescence was analyzed after 7 days. Representative images show nuclei (blue) and GFP-SQSTM1 (green). Scale bar corresponds to 20 µm. ( B ) Pooled screening workflow of GFP-SQSTM1 assay. H4 Cas9 GFP-SQSTM1 cells are transduced with lentiviral libraries of sgRNAs or shRNAs and selected for stable integration. Fluorescence activated cell sorting (FACS) is used to isolate cell populations based on GFP upper quartile fluorescence (GFP high) or GFP lower quartile fluorescence (GFP low). A representative GFP FACS histogram is shown in . Abundance <t>of</t> <t>sgRNA</t> and <t>shRNA</t> sequences is quantified by deep sequencing of the corresponding barcodes in the genomic DNA of the isolated cell populations as well as unsorted cells. ( C ) Distribution of individual sgRNAs targeting MTOR, PIK3C3, PLK1 or SQSTM1 at day 7. GFP-SQSTM1 modulation was assessed as log2 fold ratio of each sgRNA sequence based on the abundance in the GFP high versus GFP low cell population. Anti-proliferative effects were assessed as log2 fold ratio of each sgRNA based on the abundance in unsorted cells versus the input library. ( D–F ) H4 Cas9 GFP-SQSTM1 cells were transduced with CRISPR or RNAi lentiviral libraries covering 2677 genes with an average of 20 sgRNA or shRNA reagents per gene. Both screens were run in duplicate and the mean is shown. Gene-centric visualization of ( D ) average log2 fold change (FC) or ( E ) redundant siRNA activity (RSA) scores in GFP high versus GFP low cells. Selected autophagy and MTOR pathway components are highlighted in red. ( F ) Log2 fold change (FC) distribution of all shRNAs and sgRNAs in GFP high versus GFP low cells. DOI: http://dx.doi.org/10.7554/eLife.17290.002
    Custom Sgrna And Shrna Libraries, supplied by Cellecta Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/custom+sgrna+and+shrna+libraries/pmc04924995-172-7-12?v=Cellecta+Inc
    Average 90 stars, based on 1 article reviews
    custom sgrna and shrna libraries - by Bioz Stars, 2026-08
    90/100 stars

    Images

    1) Product Images from "Functional CRISPR screening identifies the ufmylation pathway as a regulator of SQSTM1/p62"

    Article Title: Functional CRISPR screening identifies the ufmylation pathway as a regulator of SQSTM1/p62

    Journal: eLife

    doi: 10.7554/eLife.17290

    ( A ) Scramble, MTOR and PIK3C3 sgRNAs were introduced into H4 Cas9 GFP-SQSTM1 cells by lentiviral infection and GFP fluorescence was analyzed after 7 days. Representative images show nuclei (blue) and GFP-SQSTM1 (green). Scale bar corresponds to 20 µm. ( B ) Pooled screening workflow of GFP-SQSTM1 assay. H4 Cas9 GFP-SQSTM1 cells are transduced with lentiviral libraries of sgRNAs or shRNAs and selected for stable integration. Fluorescence activated cell sorting (FACS) is used to isolate cell populations based on GFP upper quartile fluorescence (GFP high) or GFP lower quartile fluorescence (GFP low). A representative GFP FACS histogram is shown in . Abundance of sgRNA and shRNA sequences is quantified by deep sequencing of the corresponding barcodes in the genomic DNA of the isolated cell populations as well as unsorted cells. ( C ) Distribution of individual sgRNAs targeting MTOR, PIK3C3, PLK1 or SQSTM1 at day 7. GFP-SQSTM1 modulation was assessed as log2 fold ratio of each sgRNA sequence based on the abundance in the GFP high versus GFP low cell population. Anti-proliferative effects were assessed as log2 fold ratio of each sgRNA based on the abundance in unsorted cells versus the input library. ( D–F ) H4 Cas9 GFP-SQSTM1 cells were transduced with CRISPR or RNAi lentiviral libraries covering 2677 genes with an average of 20 sgRNA or shRNA reagents per gene. Both screens were run in duplicate and the mean is shown. Gene-centric visualization of ( D ) average log2 fold change (FC) or ( E ) redundant siRNA activity (RSA) scores in GFP high versus GFP low cells. Selected autophagy and MTOR pathway components are highlighted in red. ( F ) Log2 fold change (FC) distribution of all shRNAs and sgRNAs in GFP high versus GFP low cells. DOI: http://dx.doi.org/10.7554/eLife.17290.002
    Figure Legend Snippet: ( A ) Scramble, MTOR and PIK3C3 sgRNAs were introduced into H4 Cas9 GFP-SQSTM1 cells by lentiviral infection and GFP fluorescence was analyzed after 7 days. Representative images show nuclei (blue) and GFP-SQSTM1 (green). Scale bar corresponds to 20 µm. ( B ) Pooled screening workflow of GFP-SQSTM1 assay. H4 Cas9 GFP-SQSTM1 cells are transduced with lentiviral libraries of sgRNAs or shRNAs and selected for stable integration. Fluorescence activated cell sorting (FACS) is used to isolate cell populations based on GFP upper quartile fluorescence (GFP high) or GFP lower quartile fluorescence (GFP low). A representative GFP FACS histogram is shown in . Abundance of sgRNA and shRNA sequences is quantified by deep sequencing of the corresponding barcodes in the genomic DNA of the isolated cell populations as well as unsorted cells. ( C ) Distribution of individual sgRNAs targeting MTOR, PIK3C3, PLK1 or SQSTM1 at day 7. GFP-SQSTM1 modulation was assessed as log2 fold ratio of each sgRNA sequence based on the abundance in the GFP high versus GFP low cell population. Anti-proliferative effects were assessed as log2 fold ratio of each sgRNA based on the abundance in unsorted cells versus the input library. ( D–F ) H4 Cas9 GFP-SQSTM1 cells were transduced with CRISPR or RNAi lentiviral libraries covering 2677 genes with an average of 20 sgRNA or shRNA reagents per gene. Both screens were run in duplicate and the mean is shown. Gene-centric visualization of ( D ) average log2 fold change (FC) or ( E ) redundant siRNA activity (RSA) scores in GFP high versus GFP low cells. Selected autophagy and MTOR pathway components are highlighted in red. ( F ) Log2 fold change (FC) distribution of all shRNAs and sgRNAs in GFP high versus GFP low cells. DOI: http://dx.doi.org/10.7554/eLife.17290.002

    Techniques Used: Infection, Fluorescence, Transduction, FACS, shRNA, Sequencing, Isolation, CRISPR, Activity Assay



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    custom sgrna and shrna libraries  (Cellecta Inc)
    90
    Cellecta Inc custom sgrna and shrna libraries
    ( A ) Scramble, MTOR and PIK3C3 sgRNAs were introduced into H4 Cas9 GFP-SQSTM1 cells by lentiviral infection and GFP fluorescence was analyzed after 7 days. Representative images show nuclei (blue) and GFP-SQSTM1 (green). Scale bar corresponds to 20 µm. ( B ) Pooled screening workflow of GFP-SQSTM1 assay. H4 Cas9 GFP-SQSTM1 cells are transduced with lentiviral libraries of sgRNAs or shRNAs and selected for stable integration. Fluorescence activated cell sorting (FACS) is used to isolate cell populations based on GFP upper quartile fluorescence (GFP high) or GFP lower quartile fluorescence (GFP low). A representative GFP FACS histogram is shown in . Abundance <t>of</t> <t>sgRNA</t> and <t>shRNA</t> sequences is quantified by deep sequencing of the corresponding barcodes in the genomic DNA of the isolated cell populations as well as unsorted cells. ( C ) Distribution of individual sgRNAs targeting MTOR, PIK3C3, PLK1 or SQSTM1 at day 7. GFP-SQSTM1 modulation was assessed as log2 fold ratio of each sgRNA sequence based on the abundance in the GFP high versus GFP low cell population. Anti-proliferative effects were assessed as log2 fold ratio of each sgRNA based on the abundance in unsorted cells versus the input library. ( D–F ) H4 Cas9 GFP-SQSTM1 cells were transduced with CRISPR or RNAi lentiviral libraries covering 2677 genes with an average of 20 sgRNA or shRNA reagents per gene. Both screens were run in duplicate and the mean is shown. Gene-centric visualization of ( D ) average log2 fold change (FC) or ( E ) redundant siRNA activity (RSA) scores in GFP high versus GFP low cells. Selected autophagy and MTOR pathway components are highlighted in red. ( F ) Log2 fold change (FC) distribution of all shRNAs and sgRNAs in GFP high versus GFP low cells. DOI: http://dx.doi.org/10.7554/eLife.17290.002
    Custom Sgrna And Shrna Libraries, supplied by Cellecta Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/custom+sgrna+and+shrna+libraries/pmc04924995-172-7-12?v=Cellecta+Inc
    Average 90 stars, based on 1 article reviews
    custom sgrna and shrna libraries - by Bioz Stars, 2026-08
    90/100 stars
    		  Buy from Supplier

    Image Search Results


    ( A ) Scramble, MTOR and PIK3C3 sgRNAs were introduced into H4 Cas9 GFP-SQSTM1 cells by lentiviral infection and GFP fluorescence was analyzed after 7 days. Representative images show nuclei (blue) and GFP-SQSTM1 (green). Scale bar corresponds to 20 µm. ( B ) Pooled screening workflow of GFP-SQSTM1 assay. H4 Cas9 GFP-SQSTM1 cells are transduced with lentiviral libraries of sgRNAs or shRNAs and selected for stable integration. Fluorescence activated cell sorting (FACS) is used to isolate cell populations based on GFP upper quartile fluorescence (GFP high) or GFP lower quartile fluorescence (GFP low). A representative GFP FACS histogram is shown in . Abundance of sgRNA and shRNA sequences is quantified by deep sequencing of the corresponding barcodes in the genomic DNA of the isolated cell populations as well as unsorted cells. ( C ) Distribution of individual sgRNAs targeting MTOR, PIK3C3, PLK1 or SQSTM1 at day 7. GFP-SQSTM1 modulation was assessed as log2 fold ratio of each sgRNA sequence based on the abundance in the GFP high versus GFP low cell population. Anti-proliferative effects were assessed as log2 fold ratio of each sgRNA based on the abundance in unsorted cells versus the input library. ( D–F ) H4 Cas9 GFP-SQSTM1 cells were transduced with CRISPR or RNAi lentiviral libraries covering 2677 genes with an average of 20 sgRNA or shRNA reagents per gene. Both screens were run in duplicate and the mean is shown. Gene-centric visualization of ( D ) average log2 fold change (FC) or ( E ) redundant siRNA activity (RSA) scores in GFP high versus GFP low cells. Selected autophagy and MTOR pathway components are highlighted in red. ( F ) Log2 fold change (FC) distribution of all shRNAs and sgRNAs in GFP high versus GFP low cells. DOI: http://dx.doi.org/10.7554/eLife.17290.002

    Journal: eLife

    Article Title: Functional CRISPR screening identifies the ufmylation pathway as a regulator of SQSTM1/p62

    doi: 10.7554/eLife.17290

    Figure Lengend Snippet: ( A ) Scramble, MTOR and PIK3C3 sgRNAs were introduced into H4 Cas9 GFP-SQSTM1 cells by lentiviral infection and GFP fluorescence was analyzed after 7 days. Representative images show nuclei (blue) and GFP-SQSTM1 (green). Scale bar corresponds to 20 µm. ( B ) Pooled screening workflow of GFP-SQSTM1 assay. H4 Cas9 GFP-SQSTM1 cells are transduced with lentiviral libraries of sgRNAs or shRNAs and selected for stable integration. Fluorescence activated cell sorting (FACS) is used to isolate cell populations based on GFP upper quartile fluorescence (GFP high) or GFP lower quartile fluorescence (GFP low). A representative GFP FACS histogram is shown in . Abundance of sgRNA and shRNA sequences is quantified by deep sequencing of the corresponding barcodes in the genomic DNA of the isolated cell populations as well as unsorted cells. ( C ) Distribution of individual sgRNAs targeting MTOR, PIK3C3, PLK1 or SQSTM1 at day 7. GFP-SQSTM1 modulation was assessed as log2 fold ratio of each sgRNA sequence based on the abundance in the GFP high versus GFP low cell population. Anti-proliferative effects were assessed as log2 fold ratio of each sgRNA based on the abundance in unsorted cells versus the input library. ( D–F ) H4 Cas9 GFP-SQSTM1 cells were transduced with CRISPR or RNAi lentiviral libraries covering 2677 genes with an average of 20 sgRNA or shRNA reagents per gene. Both screens were run in duplicate and the mean is shown. Gene-centric visualization of ( D ) average log2 fold change (FC) or ( E ) redundant siRNA activity (RSA) scores in GFP high versus GFP low cells. Selected autophagy and MTOR pathway components are highlighted in red. ( F ) Log2 fold change (FC) distribution of all shRNAs and sgRNAs in GFP high versus GFP low cells. DOI: http://dx.doi.org/10.7554/eLife.17290.002

    Article Snippet: For the focused libraries, custom sgRNA and shRNA libraries were constructed by Cellecta as previously described ( ).

    Techniques: Infection, Fluorescence, Transduction, FACS, shRNA, Sequencing, Isolation, CRISPR, Activity Assay